Real Time PCR identification of animal species of fisheries origin traded in Argentina

Abstract

The wide diversity of species used for the processing of fishery products makes necessary the development of a versatile analytical methodology that allows the control of labeling, adulteration and/or contamination in raw materials and processed products. In the identification of species using the Real Time PCR technique, the high specificity and stability of the DNA molecule against the thermal treatments used in processing make it a reliable and objective control tool.

The objective of the present work was to establish a methodology for the identification of the most commercially important fish species in Argentina through the design of specific primers and probes, using bioinformatics tools and the development of a Real Time PCR analysis methodology. For most of the species studied, the method developed allowed their correct identification with high sensitivity and specificity. Some interferences were found in the analysis of southern hake, skipjack tuna and mackerel. The results obtained will allow the possibility of evaluating the authenticity of fishery products made from the species studied.